Nikon LiveScan Swept Field Confocal Microscope

Whether you're doing multi-dimensional imaging of cells (X,Y,Z,T or even X,Y,Z,T at multiple coordinates), observing fast neural events, or looking to capture live-motion images of cells, the Nikon LiveScan SFC (Swept Field Confocal) microscope offers advance technology for your advanced research.

The key to the image quality is Nikon's patent-pending swept field technology.

Unlike confocal systems with pinhole apertures embedded in a spinning disc, the Nikon LiveScan SFC's 32 pinhole array remains stationary. Galvonometric and piezo controlled mirrors sweep the image of the pinholes across the sample. The emission photons are de-scanned and focused through a complementary set of pinholes onto a CCD camera

This swept field technology overcomes some limitations of systems with spinning discs such as a lack of synchronization between the moving pinholes and the CCD camera, crosstalk between pinholes, and true confocality with a very limited selection of objective lenses. Another common problem with spinning disc systems is that banding occurs at rapid frame rates. The Nikon LiveScan SFC has the fastest rates of data acquisition of any commercially available confocal system.

Also, in contrast to systems with spinning discs, where the pinhole diameter is set, Nikon LiveScan SFC offers four selectable confocal pinhole diameters (from 30 microns to 90 microns), along with a slit scanning (35 and 70 microns) mode- so you can choose to optimize sensitivity, resolution, or speed.

The LiveScan SFC offers you:

• Speed: the fastest data acquisition speeds of any commercially available confocal system
  • Maximum acquisition frame rate exceeding 1000frames/second inslit scan mode
  • up to 120 frames/second in pinhole mode for high resolution
• High Efficiency Optical System: Swept field technology and state-of-the art optics yield a high rate of photon capture, in the range of 440-700nm
• Multiple Laser Configuration Options
• Multiple Wavelength Scanning: possibility of up to 6 wavelengths in one fiber and single or dual fiber outputs
• Compatible with TIRF Illumination

Why settle for spinning disk when you can have Swept Field? Seeing is believing. Click here to request a demonstration.

To find out more about how the Nikon LiveScan SFC is bringing live-cell image capture to a new level, or to discuss what configuration is best suited for your research, call 1-800-570-4277

One-pass, high-resolution, wide-band spectral imaging

• One-Pass:

  • Simultaneously acquire 32 channels of fluorescence spectra in a single pass. The C1si has more useful data channels than any other confocal system.

  • The speed of the one-pass system minimizes damage to living cells, reduces photo-bleaching, and enhances the quality of time-lapsed imaging.
• High Resolution: Select 2nm, 5nm, or 10nm channel width.
• Wide-Band Acquisition: Spectra over a full range of 320nm (400nm-750nm) can be obtained in a single pass, at 10nm.

• Proprietary optics (patent pending) that very efficiently capture fluorescence emission photons.
• Signal-processing circuitry (patent pending) that digitizes for the full pixel period, eliminating dead time.

• True fluorescence colors across spectrum.
• Accurate fluorescence spectrum even at highest resolution (2 nm).

• Observing multistained specimens
• FRET experiments
• Separating the signal from autofluorescence

Fluorescence spectra of GFP and Alexa 488 Spectra displayed at 2.5 nm wavelength resolution per channel of the fluorescence detector in the 493-570.5 nm range.	Separation of GFP and Alexa 488 spectra GFP expressed in HeLa cell nuclei and actin stained with Alexa 488. Excitation wavelength 488 nm.] Combined channel True Color image.obtained with 2.5 nm wavelength resolution in 493-570.5 nm range.   Image with separated spectra after using unmixing software.

Unmixing red fluorochromes Red flourochromes,which had previously posed a challenge, are now simple to unmix as well. Rhodamine's fluorescence spectral peak is at approximately 579 nm, while that for RFP is approximately 600 nm. RFP's fluorescence is weaker than Rhodamine's, but their spectra are cleanly unmixed.     RFP expressed in HeLa cell nuclei and actin stained with Rhodamine. True Color image overlay of 32 images in the 550-630 nm range at 2.5 nm wavelength resolution. After fluorescence unmixing.

Compatible with TIRF Illumination
Easy To Operate. Easy to Modify.

• Switch easily:
  • from the high-resolution, 32 channel detector to a standard 3 channel confocal detector with all the optical advances of the C1 Plus.
  • from eyepiece observation to laser scanning mode.
• Variable Color Filtering: Wavelength information from the entire range can be gathered in one pass. Afterward, you can easily digitally filter in any desired wavelength range.
• Intuitive Software: Most parameters can be modified with a single click of the mouse.